Training Modules

Centrifugation Theory and Practice     
• Routine centrifuge rotors
• Calculation of g-force
• Differential centrifugation
• Density gradient theory
 
Read more... 
[ You will need to login or create an account to access this content ]

Density Gradients     
• Modern iodinated density gradient media
• Making pre-formed discontinuous and continuous gradients
• Modern rotor technology for density gradients
• Self-generated gradients
• Harvesting gradients 

Read more...
[ You will need to login or create an account to access this content ]

Purification of blood leukocytes     
• Description of media
• Handling general purpose media such as OptiPrep™
• Use of commercial media customized to a specific blood cell isolation
• Use of general purpose media such as OptiPrep™
 
Read more...
[ You will need to login or create an account to access this content ]

Virus Purification     
• Pre-gradient treatment: ammonium sulphate precipitation; cellufine-SO4 chromatography
• Density gradient centrifugation
• Post-gradient add-on processes: e.g. HPLC
• Re-infection of cells
• Electrophoresis

Read more...
[ You will need to login or create an account to access this content ]

Purification of subcellular organelles     
• Typical strategy for the fractionation of a light mitochondrial fraction
• Discontinuous gradients for the isolation of specific organelles

Read more...
[ You will need to login or create an account to access this content ]